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anti hexa his tag mouse igg mab  (Sino Biological)
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Anti Hexa His Tag Mouse Igg Mab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hexa his tag  (GE Healthcare)
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monoclonal anti hexa his antibody  (Santa Cruz Biotechnology)
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Inoculation of HepG2- and differentiated HepaRG cells by exosomal HBV and free HBV. A , HBV genome copy number in each exosomal and viral inoculum is shown in the left . Schematic description of the infection experiment procedure is shown in the right . B , HBsAg ELISA of supernatant derived from HepG2 cells inoculated either with exosomal HBV virions or free HBV virions. Medium was changed at the indicated time points and analyzed by HBsAg ELISA. The horizontal dotted line indicated the cutoff value. C , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated either with exosomal HBV (fraction 8 of the iodixanol gradient) or a less pure, later exosomal fraction (fraction 10). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific <t>monoclonal</t> antibody (1 μg/mL) as control. Medium was changed at the indicated time points and analyzed by HBsAg-specific ELISA. S/CO, Sample to cutoff signal. The cutoff value is represented by the horizontal dotted line . D , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated with free HBV virions (fractions 13 and 14). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific monoclonal (1 μg/mL) as control. Medium was changed at the indicated time points and analyzed by HBsAg-specific ELISA. The horizontal dotted line delineates the cutoff value. E , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated either with exosomal HBV (fractions 7, 8 of the iodixanol gradient) or a less pure, later exosomal fraction (fractions 9, 10 of the iodixanol gradient). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific monoclonal (1 μg/mL) as control. Here, the HBsAg content at day 4 of the different samples is analyzed. Unpaired parametric t tests for all panels; ∗∗ P < .01; ∗∗∗ P < .001; ns, not significant. F–G , Differentiated HepaRG cells were inoculated with free HBV virions (fractions 13) ( F ) or exosomal HBV (fraction 8 of the iodixanol gradient) ( G ), and secreted HBsAg was quantified at the indicated time points by HBsAg-specific ELISA. Myrcludex B 500 nM was added during infection. The horizontal dotted line indicates the cutoff value.
Monoclonal Anti Hexa His Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti-hexa-his-tag dia-900 antibody  (Biozol Diagnostica Vertrieb GmbH)
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Biozol Diagnostica Vertrieb GmbH mouse anti-hexa-his-tag dia-900 antibody
Inoculation of HepG2- and differentiated HepaRG cells by exosomal HBV and free HBV. A , HBV genome copy number in each exosomal and viral inoculum is shown in the left . Schematic description of the infection experiment procedure is shown in the right . B , HBsAg ELISA of supernatant derived from HepG2 cells inoculated either with exosomal HBV virions or free HBV virions. Medium was changed at the indicated time points and analyzed by HBsAg ELISA. The horizontal dotted line indicated the cutoff value. C , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated either with exosomal HBV (fraction 8 of the iodixanol gradient) or a less pure, later exosomal fraction (fraction 10). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific <t>monoclonal</t> antibody (1 μg/mL) as control. Medium was changed at the indicated time points and analyzed by HBsAg-specific ELISA. S/CO, Sample to cutoff signal. The cutoff value is represented by the horizontal dotted line . D , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated with free HBV virions (fractions 13 and 14). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific monoclonal (1 μg/mL) as control. Medium was changed at the indicated time points and analyzed by HBsAg-specific ELISA. The horizontal dotted line delineates the cutoff value. E , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated either with exosomal HBV (fractions 7, 8 of the iodixanol gradient) or a less pure, later exosomal fraction (fractions 9, 10 of the iodixanol gradient). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific monoclonal (1 μg/mL) as control. Here, the HBsAg content at day 4 of the different samples is analyzed. Unpaired parametric t tests for all panels; ∗∗ P < .01; ∗∗∗ P < .001; ns, not significant. F–G , Differentiated HepaRG cells were inoculated with free HBV virions (fractions 13) ( F ) or exosomal HBV (fraction 8 of the iodixanol gradient) ( G ), and secreted HBsAg was quantified at the indicated time points by HBsAg-specific ELISA. Myrcludex B 500 nM was added during infection. The horizontal dotted line indicates the cutoff value.
Mouse Anti Hexa His Tag Dia 900 Antibody, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alexa-555 conjugated anti-hexa-his tag antibody  (Thermo Fisher)
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Thermo Fisher alexa-555 conjugated anti-hexa-his tag antibody
Inoculation of HepG2- and differentiated HepaRG cells by exosomal HBV and free HBV. A , HBV genome copy number in each exosomal and viral inoculum is shown in the left . Schematic description of the infection experiment procedure is shown in the right . B , HBsAg ELISA of supernatant derived from HepG2 cells inoculated either with exosomal HBV virions or free HBV virions. Medium was changed at the indicated time points and analyzed by HBsAg ELISA. The horizontal dotted line indicated the cutoff value. C , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated either with exosomal HBV (fraction 8 of the iodixanol gradient) or a less pure, later exosomal fraction (fraction 10). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific <t>monoclonal</t> antibody (1 μg/mL) as control. Medium was changed at the indicated time points and analyzed by HBsAg-specific ELISA. S/CO, Sample to cutoff signal. The cutoff value is represented by the horizontal dotted line . D , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated with free HBV virions (fractions 13 and 14). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific monoclonal (1 μg/mL) as control. Medium was changed at the indicated time points and analyzed by HBsAg-specific ELISA. The horizontal dotted line delineates the cutoff value. E , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated either with exosomal HBV (fractions 7, 8 of the iodixanol gradient) or a less pure, later exosomal fraction (fractions 9, 10 of the iodixanol gradient). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific monoclonal (1 μg/mL) as control. Here, the HBsAg content at day 4 of the different samples is analyzed. Unpaired parametric t tests for all panels; ∗∗ P < .01; ∗∗∗ P < .001; ns, not significant. F–G , Differentiated HepaRG cells were inoculated with free HBV virions (fractions 13) ( F ) or exosomal HBV (fraction 8 of the iodixanol gradient) ( G ), and secreted HBsAg was quantified at the indicated time points by HBsAg-specific ELISA. Myrcludex B 500 nM was added during infection. The horizontal dotted line indicates the cutoff value.
Alexa 555 Conjugated Anti Hexa His Tag Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hexa  (Cell Signaling Technology Inc)
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Inoculation of HepG2- and differentiated HepaRG cells by exosomal HBV and free HBV. A , HBV genome copy number in each exosomal and viral inoculum is shown in the left . Schematic description of the infection experiment procedure is shown in the right . B , HBsAg ELISA of supernatant derived from HepG2 cells inoculated either with exosomal HBV virions or free HBV virions. Medium was changed at the indicated time points and analyzed by HBsAg ELISA. The horizontal dotted line indicated the cutoff value. C , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated either with exosomal HBV (fraction 8 of the iodixanol gradient) or a less pure, later exosomal fraction (fraction 10). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific <t>monoclonal</t> antibody (1 μg/mL) as control. Medium was changed at the indicated time points and analyzed by HBsAg-specific ELISA. S/CO, Sample to cutoff signal. The cutoff value is represented by the horizontal dotted line . D , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated with free HBV virions (fractions 13 and 14). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific monoclonal (1 μg/mL) as control. Medium was changed at the indicated time points and analyzed by HBsAg-specific ELISA. The horizontal dotted line delineates the cutoff value. E , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated either with exosomal HBV (fractions 7, 8 of the iodixanol gradient) or a less pure, later exosomal fraction (fractions 9, 10 of the iodixanol gradient). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific monoclonal (1 μg/mL) as control. Here, the HBsAg content at day 4 of the different samples is analyzed. Unpaired parametric t tests for all panels; ∗∗ P < .01; ∗∗∗ P < .001; ns, not significant. F–G , Differentiated HepaRG cells were inoculated with free HBV virions (fractions 13) ( F ) or exosomal HBV (fraction 8 of the iodixanol gradient) ( G ), and secreted HBsAg was quantified at the indicated time points by HBsAg-specific ELISA. Myrcludex B 500 nM was added during infection. The horizontal dotted line indicates the cutoff value.
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mouse anti hexa his tag primary antibody  (Proteintech)
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Inoculation of HepG2- and differentiated HepaRG cells by exosomal HBV and free HBV. A , HBV genome copy number in each exosomal and viral inoculum is shown in the left . Schematic description of the infection experiment procedure is shown in the right . B , HBsAg ELISA of supernatant derived from HepG2 cells inoculated either with exosomal HBV virions or free HBV virions. Medium was changed at the indicated time points and analyzed by HBsAg ELISA. The horizontal dotted line indicated the cutoff value. C , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated either with exosomal HBV (fraction 8 of the iodixanol gradient) or a less pure, later exosomal fraction (fraction 10). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific <t>monoclonal</t> antibody (1 μg/mL) as control. Medium was changed at the indicated time points and analyzed by HBsAg-specific ELISA. S/CO, Sample to cutoff signal. The cutoff value is represented by the horizontal dotted line . D , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated with free HBV virions (fractions 13 and 14). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific monoclonal (1 μg/mL) as control. Medium was changed at the indicated time points and analyzed by HBsAg-specific ELISA. The horizontal dotted line delineates the cutoff value. E , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated either with exosomal HBV (fractions 7, 8 of the iodixanol gradient) or a less pure, later exosomal fraction (fractions 9, 10 of the iodixanol gradient). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific monoclonal (1 μg/mL) as control. Here, the HBsAg content at day 4 of the different samples is analyzed. Unpaired parametric t tests for all panels; ∗∗ P < .01; ∗∗∗ P < .001; ns, not significant. F–G , Differentiated HepaRG cells were inoculated with free HBV virions (fractions 13) ( F ) or exosomal HBV (fraction 8 of the iodixanol gradient) ( G ), and secreted HBsAg was quantified at the indicated time points by HBsAg-specific ELISA. Myrcludex B 500 nM was added during infection. The horizontal dotted line indicates the cutoff value.
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Inoculation of HepG2- and differentiated HepaRG cells by exosomal HBV and free HBV. A , HBV genome copy number in each exosomal and viral inoculum is shown in the left . Schematic description of the infection experiment procedure is shown in the right . B , HBsAg ELISA of supernatant derived from HepG2 cells inoculated either with exosomal HBV virions or free HBV virions. Medium was changed at the indicated time points and analyzed by HBsAg ELISA. The horizontal dotted line indicated the cutoff value. C , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated either with exosomal HBV (fraction 8 of the iodixanol gradient) or a less pure, later exosomal fraction (fraction 10). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific monoclonal antibody (1 μg/mL) as control. Medium was changed at the indicated time points and analyzed by HBsAg-specific ELISA. S/CO, Sample to cutoff signal. The cutoff value is represented by the horizontal dotted line . D , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated with free HBV virions (fractions 13 and 14). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific monoclonal (1 μg/mL) as control. Medium was changed at the indicated time points and analyzed by HBsAg-specific ELISA. The horizontal dotted line delineates the cutoff value. E , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated either with exosomal HBV (fractions 7, 8 of the iodixanol gradient) or a less pure, later exosomal fraction (fractions 9, 10 of the iodixanol gradient). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific monoclonal (1 μg/mL) as control. Here, the HBsAg content at day 4 of the different samples is analyzed. Unpaired parametric t tests for all panels; ∗∗ P < .01; ∗∗∗ P < .001; ns, not significant. F–G , Differentiated HepaRG cells were inoculated with free HBV virions (fractions 13) ( F ) or exosomal HBV (fraction 8 of the iodixanol gradient) ( G ), and secreted HBsAg was quantified at the indicated time points by HBsAg-specific ELISA. Myrcludex B 500 nM was added during infection. The horizontal dotted line indicates the cutoff value.

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Presence of Intact Hepatitis B Virions in Exosomes

doi: 10.1016/j.jcmgh.2022.09.012

Figure Lengend Snippet: Inoculation of HepG2- and differentiated HepaRG cells by exosomal HBV and free HBV. A , HBV genome copy number in each exosomal and viral inoculum is shown in the left . Schematic description of the infection experiment procedure is shown in the right . B , HBsAg ELISA of supernatant derived from HepG2 cells inoculated either with exosomal HBV virions or free HBV virions. Medium was changed at the indicated time points and analyzed by HBsAg ELISA. The horizontal dotted line indicated the cutoff value. C , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated either with exosomal HBV (fraction 8 of the iodixanol gradient) or a less pure, later exosomal fraction (fraction 10). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific monoclonal antibody (1 μg/mL) as control. Medium was changed at the indicated time points and analyzed by HBsAg-specific ELISA. S/CO, Sample to cutoff signal. The cutoff value is represented by the horizontal dotted line . D , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated with free HBV virions (fractions 13 and 14). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific monoclonal (1 μg/mL) as control. Medium was changed at the indicated time points and analyzed by HBsAg-specific ELISA. The horizontal dotted line delineates the cutoff value. E , HBsAg ELISA of supernatant derived from differentiated HepaRG cells inoculated either with exosomal HBV (fractions 7, 8 of the iodixanol gradient) or a less pure, later exosomal fraction (fractions 9, 10 of the iodixanol gradient). The inoculum was preincubated with the LHBs-specific antibody MA18/7 (1 μg/mL) or an anti-hexa-His-specific monoclonal (1 μg/mL) as control. Here, the HBsAg content at day 4 of the different samples is analyzed. Unpaired parametric t tests for all panels; ∗∗ P < .01; ∗∗∗ P < .001; ns, not significant. F–G , Differentiated HepaRG cells were inoculated with free HBV virions (fractions 13) ( F ) or exosomal HBV (fraction 8 of the iodixanol gradient) ( G ), and secreted HBsAg was quantified at the indicated time points by HBsAg-specific ELISA. Myrcludex B 500 nM was added during infection. The horizontal dotted line indicates the cutoff value.

Article Snippet: To the purified HBV exosomal fractions or free HBV virus fractions, the RBD-binding anti-preS1 antibody (MA18/7, 1 μg/mL) or an unrelated monoclonal anti-hexa-His antibody (sc-8036, Santa Cruz) was added and kept at 37 °C for 2 hours.

Techniques: Infection, Enzyme-linked Immunosorbent Assay, Derivative Assay, Control